A novel genotype-independent technique for successful induction of somatic embryogenesis of adult plants of Jatropha curcas L. using petiole transverse Thin Cell Layer (TCL)

Developing a protocol for somatic embryogenesis (SE) adult mother plants of Jatropha curcas, aids to overcome problems such as harvesting time and an uneven yield, providing the opportunity propagate proven elite genotypes. Until now, several authors have achieved SE in J. curcas using different explants, however, none these protocols been used plant material. Furthermore, challenge overcoming morphogenesis limitations successfully further regeneration has great. The main objective this study was develop oil producing curcas. transverse thin cell layers (tTCL) young petioles were explants. Half strength Y3 MS media containing three concentrations 2,4-dichloropherioxyacetic acid 6-benzil-aminopurine factorial trial. Nine accessions various ages (3-12 years.) tested protocol. All responded positively callus induction embryos, demonstrating be genotype-independent. This enables micropropagation via embryogenesis.    Key words: (tTCL), half media, regeneration.